Sphingomyelinase Treatment Induces ATP-independent Endocytosis

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Sphingomyelinase Treatment Induces ATP-independent Endocytosis

ATP hydrolysis has been regarded as a general requirement for internalization processes in mammalian cells. We found, however, that treatment of ATP-depleted macrophages and fibroblasts with exogenous sphingomyelinase (SMase) rapidly induces formation of numerous vesicles that pinch off from the plasma membrane; the process is complete within 10 min after adding SMase. By electron microscopy, t...

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ژورنال

عنوان ژورنال: Journal of Cell Biology

سال: 1998

ISSN: 0021-9525,1540-8140

DOI: 10.1083/jcb.140.1.39